目的 研究淡豆豉 (Semen Sojae Praeparatum) 的化学成分及其促进大鼠颅骨细胞增殖的活性。方法 采用反复硅胶柱色谱、MCI和Sephadex LH-20柱色谱等方法进行分离纯化,并通过化合物的理化性质和波谱数据进行结构鉴定。采用MTT法考察化合物对大鼠颅骨细胞增殖的作用。结果 分离得到12个化合物,其中7个黄酮化合物:大豆苷(1),大豆苷元(2),染料木素(3),染料木苷(4),黄豆黄素(5),黄豆黄苷(6),芹菜素(7);3个甾类化合物:β-谷甾醇(8),豆甾醇(9),菜油甾醇(10);2个小分子芳香化合物:丁香醛(11),丁香酸(12)。结论 化合物5~11均为首次从淡豆豉中分离得到。5个异黄酮类化合物具有一定的促成骨细胞增殖活性,且在1×10-9~1×10-6mol·L-1内细胞增殖呈现明显的剂量依赖,3个黄酮苷元(大豆苷元、染料木素、黄豆黄素)的促成骨细胞增殖活性优于2个黄酮苷(大豆苷、染料木苷)。
Abstract
OBJECTIVE To study the chemical constituents and their proliferation activity on rat osteoblasts of Semen Sojae Praeparatum. METHODS The compounds were isolated by chromatography on silica gel column,MCI and sephadex LH-20 column. Their structures were elucidated by spectral analysis. The proliferation test was performed by using rat osteoblasts with MTT assays in vitro. RESULTS Twelve compounds were obtained and identified as daidzin(1), daidzein(2),genistein(3), genistin(4), glycitein(5), glycitin(6),apigenin(7), β-sitosterol(8), stigmasterol(9), campesterol(10), syringaldehyde(11), syringic acid(12), respectively. CONCLUSION Compounds 5-11 are isolated from Semen Sojae Praeparatum for the first time. The five isoflavones show proliferation activity on rat osteoblasts, and aglycones proved to show better activity than glycosides.
关键词
淡豆豉 /
黄豆黄素 /
黄豆黄苷 /
芹菜素 /
分离纯化 /
结构鉴定
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Key words
Semen Sojae Praeparatum /
glycitein /
glycitin /
apigenin /
isolation purification /
structure identification
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中图分类号：
R284
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参考文献
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脚注
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基金
河北省自然科学基金资助项目(H2012206019,H2014206302)
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